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KMID : 0613820150250010062
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Journal of Life Science
2015 Volume.25 No. 1 p.62 ~ p.67
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Effects of Myelophycus Simplex Papenfuss Methanol Extract on Adipocyte Differentiation and Adipogenesis in 3T3-L1 Preadipocytes
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Kim Hyang-Suk
Kwon Da-Hye
Cheon Ji-Min
Choi Eun-Ok
Kim Ji-Hyun
Han Min-Ho
Choi Young-Hyun
Kim Byung-Woo
Hwang Hye-Jin
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Abstract
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Myelophycus simplex Papenfuss is distributed over the northern Pacific and southern coast of Korea, and is a member of the brown algae family. The objective of this study was to investigate the effect of M. simplex methanol extract on adipocyte differentiation and adipogenesis in 3T3-L1 preadipocytes. Treatment with M. simplex methanol extract significantly suppressed terminal differentiation of 3T3-L1 preadipocytes in a dose-dependent manner, as confirmed by a decrease in lipid droplet content observed by Oil Red O staining. Also, the M. simplex methanol extract significantly suppressed the triglyceride content of 3T3-L1 preadipocytes in a dose-dependent manner. Treatment with 300 and 500 μg/ml of M. simplex methanol extract caused a 42% and 76% reduction in lipid droplet content, respectively. In order to understand the anti-adipogenic effects of M. simplex methanol extract, the changes in the expression of several adipogenic transcription factors, including peroxisome proliferator-activated receptor (PPAR) γ-cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT) /enhancer binding protein (C/EBP) α and β, were investigated using immunoblotting. M. simplex suppressed the expression of PPARγ, C/EBPα, and C/EBPβ proteins compared with control. Therefore, the results of this study suggest that M. simplex methanol extract inhibits adipocyte differentiation and thus may have applications as a potential source for an anti-obesity functional food agent.
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KEYWORD
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Adipogenesis, Myelophycus simplex Papenfuss, triglyceride, 3T3-L1
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